Anna Wu
Professor, Molecular & Medical Pharmacology
Faculty, Crump Institute for Molecular Imaging
Member, DOE Funded Faculty, JCCC Genitourinary Oncology Program Area

Molecular approaches to detection and treatment of cancer

Work Email Address:  awu@mednet.ucla.edu
Home Page: http://faculty.pharmacology.ucla.edu/institution/personnel?personnel_id=45665
Laboratory Address:
Crump Institute
Office Address:
CNSI 4335, Crump Institute,570 Westwood Plaza


Phone Numbers:
(310) 794-5088

Selected Publications:

Wilks Moses Q, Knowles Scott M, Wu Anna M, Huang Sung-Cheng Improved Modeling of In Vivo Kinetics of Slowly Diffusing Radiotracers for Tumor Imaging. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 2014; .
Viola-Villegas Nerissa Therese, Sevak Kuntal K, Carlin Sean D, Doran Michael G, Evans Henry W, Bartlett Derek W, Wu Anna M, Lewis Jason S Noninvasive Imaging of PSMA in Prostate Tumors with (89)Zr-Labeled huJ591 Engineered Antibody Fragments: The Faster Alternatives. Molecular pharmaceutics. 2014; 16(3): .
Rochefort Matthew M, Girgis Mark D, Knowles Scott M, Ankeny Jacob S, Salazar Felix, Wu Anna M, Tomlinson James S A Mutated Anti-CA19-9 scFv-Fc for Positron Emission Tomography of Human Pancreatic Cancer Xenografts. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging. 2014; 5(4): .
Watanabe Rira, Sato Kazuhide, Hanaoka Hirofumi, Harada Toshiko, Nakajima Takahito, Kim Insook, Paik Chang H, Wu Anna M, Choyke Peter L, Kobayashi Hisataka Minibody-indocyanine green based activatable optical imaging probes: the role of short polyethylene glycol linkers. ACS medicinal chemistry letters. 2014; 5(4): 411-5.
Williams Lawrence E, Wu Anna M, Kenanova Vania E, Olafsen Tove, Yazaki Paul J Numerical comparison of iodine-based and indium-based antibody biodistributions. Cancer biotherapy & radiopharmaceuticals. 2014; 29(3): 91-8.
Knowles Scott M, Zettlitz Kirstin A, Tavaré Richard, Rochefort Matthew M, Salazar Felix B, Stout David B, Yazaki Paul J, Reiter Robert E, Wu Anna M Quantitative immunoPET of prostate cancer xenografts with 89Zr- and 124I-labeled anti-PSCA A11 minibody. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 2014; 55(3): 452-9.
Wu Anna M Engineered antibodies for molecular imaging of cancer. Methods (San Diego, Calif.). 2014; 65(1): 139-47.
Tavaré Richard, McCracken Melissa N, Zettlitz Kirstin A, Knowles Scott M, Salazar Felix B, Olafsen Tove, Witte Owen N, Wu Anna M Engineered antibody fragments for immuno-PET imaging of endogenous CD8+ T cells in vivo. Proceedings of the National Academy of Sciences of the United States of America. 2014; 111(3): 1108-13.
Girgis Mark D, Federman Noah, Rochefort Matthew M, McCabe Katelyn E, Wu Anna M, Nagy Jon O, Denny Christopher, Tomlinson James S An engineered anti-CA19-9 cys-diabody for positron emission tomography imaging of pancreatic cancer and targeting of polymerized liposomal nanoparticles. The Journal of surgical research. 2013; 185(1): 45-55.
Widney Daniel P, Olafsen Tove, Wu Anna M, Kitchen Christina M R, Said Jonathan W, Smith Jeffrey B, Peña Guadalupe, Magpantay Larry I, Penichet Manuel L, Martinez-Maza Otoniel Levels of murine, but not human, CXCL13 are greatly elevated in NOD-SCID mice bearing the AIDS-associated Burkitt lymphoma cell line, 2F7. PloS one. 2013; 8(8): e72414.
Knowles Scott M, Wu Anna M Advances in immuno-positron emission tomography: antibodies for molecular imaging in oncology. Journal of clinical oncology : official journal of the American Society of Clinical Oncology. 2012; 30(31): 3884-92.
Andersen Jan Terje, Foss Stian, Kenanova Vania E, Olafsen Tove, Leikfoss Ingvild S, Roopenian Derry C, Wu Anna M, Sandlie Inger Anti-carcinoembryonic antigen single-chain variable fragment antibody variants bind mouse and human neonatal Fc receptor with different affinities that reveal distinct cross-species differences in serum half-life. The Journal of biological chemistry. 2012; 287(27): 22927-37.
Fu Maoyong, Brewer Sarah, Olafsen Tove, Wu Anna M, Gordon Lynn K, Said Jonathan, Braun Jonathan, Wadehra Madhuri Positron Emission Tomography Imaging of Endometrial Cancer Using Engineered Anti-EMP2 Antibody Fragments. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging. 2012; 36(3): .
Olafsen Tove, Sirk Shannon J, Olma Sebastian, Shen Clifton K-F, Wu Anna M ImmunoPET using engineered antibody fragments: fluorine-18 labeled diabodies for same-day imaging. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2012; 132(2): .
Federman Noah, Chan Jason, Nagy Jon O, Landaw Elliot M, McCabe Katelyn, Wu Anna M, Triche Timothy, Kang Hyunggyoo, Liu Bin, Marks James D, Denny Christopher T Enhanced growth inhibition of osteosarcoma by cytotoxic polymerized liposomal nanoparticles targeting the alcam cell surface receptor. Sarcoma. 2012; 2012(2): 126906.
Girgis Mark D, Olafsen Tove, Kenanova Vania, McCabe Katelyn E, Wu Anna M, Tomlinson James S Targeting CEA in Pancreas Cancer Xenografts with a Mutated scFv-Fc Antibody Fragment. EJNMMI research. 2011; 1(1): 24.
Girgis Mark D, Kenanova Vania, Olafsen Tove, McCabe Katelyn E, Wu Anna M, Tomlinson James S Anti-CA19-9 diabody as a PET imaging probe for pancreas cancer. The Journal of surgical research. 2011; 170(2): 169-78.
Liu Kan, Wang Ming-Wei, Lin Wei-Yu, Phung Duy Linh, Girgis Mark D, Wu Anna M, Tomlinson James S, Shen Clifton K-F Molecular Imaging Probe Development using Microfluidics. Current organic synthesis. 2011; 8(4): 473-487.
Olafsen Tove, Elgqvist Jorgen, Wu Anna M Protein targeting constructs in alpha therapy. Current radiopharmaceuticals. 2011; 4(3): 197-213.
McCabe Katelyn E, Liu Bin, Marks James D, Tomlinson James S, Wu Hong, Wu Anna M An Engineered Cysteine-Modified Diabody for Imaging Activated Leukocyte Cell Adhesion Molecule (ALCAM)-Positive Tumors. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging. 2011; 10(3): .
Barat Bhaswati, Kenanova Vania E, Olafsen Tove, Wu Anna M Evaluation of two internalizing carcinoembryonic antigen reporter genes for molecular imaging. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging. 2011; 13(3): 526-35.
Liu Kan, Lepin Eric J, Wang Ming-Wei, Guo Feng, Lin Wei-Yu, Chen Yi-Chun, Sirk Shannon J, Olma Sebastian, Phelps Michael E, Zhao Xing-Zhong, Tseng Hsian-Rong, Michael van Dam R, Wu Anna M, Shen Clifton K-F Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography. Molecular imaging. 2011; 10(3): 168-76, 1-7.
Morizono Kouki, Xie Yiming, Olafsen Tove, Lee Benhur, Dasgupta Asim, Wu Anna M, Chen Irvin S Y The soluble serum protein Gas6 bridges virion envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry. Cell host & microbe. 2011; 9(4): 286-98.
Girgis Mark D, Olafsen Tove, Kenanova Vania, McCabe Katelyn E, Wu Anna M, Tomlinson James S CA19-9 as a Potential Target for Radiolabeled Antibody-Based Positron Emission Tomography of Pancreas Cancer. International journal of molecular imaging. 2011; 2011(1): 834515.
Olafsen Tove, Young Stephen G, Davies Brandon S J, Beigneux Anne P, Kenanova Vania E, Voss Constance, Young Glen, Wong Koon-Pong, Barnes Richard H, Tu Yiping, Weinstein Michael M, Nobumori Chika, Huang Sung-Cheng, Goldberg Ira J, Bensadoun André, Wu Anna M, Fong Loren G Unexpected expression pattern for glycosylphosphatidylinositol-anchored HDL-binding protein 1 (GPIHBP1) in mouse tissues revealed by positron emission tomography scanning. The Journal of biological chemistry. 2010; 285(50): 39239-48.
Kenanova Vania E, Olafsen Tove, Salazar Felix B, Williams Lawrence E, Knowles Scott, Wu Anna M Tuning the serum persistence of human serum albumin domain III:diabody fusion proteins. Protein engineering, design & selection : PEDS. 2010; 23(10): 789-98.
Wong Jeffrey Y C, Raubitschek Andrew, Yamauchi Dave, Williams Lawrence E, Wu Anna M, Yazaki Paul, Shively John E, Colcher David, Somlo George A pretherapy biodistribution and dosimetry study of indium-111-radiolabeled trastuzumab in patients with human epidermal growth factor receptor 2-overexpressing breast cancer. Cancer biotherapy & radiopharmaceuticals. 2010; 25(4): 387-94.
Lepin Eric J, Leyton Jeffrey V, Zhou Yu, Olafsen Tove, Salazar Felix B, McCabe Katelyn E, Hahm Scott, Marks James D, Reiter Robert E, Wu Anna M An affinity matured minibody for PET imaging of prostate stem cell antigen (PSCA)-expressing tumors. European journal of nuclear medicine and molecular imaging. 2010; 37(8): 1529-38.
Wong JY, Raubitschek A, Yamauchi D, Williams LE, Wu AM, Yazaki P, Shively JE, Colcher D, Somlo G A pretherapy biodistribution and dosimetry study of indium-111-radiolabeled trastuzumab in patients with human epidermal growth factor receptor 2-overexpressing breast cancer. Cancer Biother Radiopharm. 2010; 25(4): 387-94.
Lepin EJ, Leyton JV, Zhou Y, Olafsen T, Salazar FB, McCabe KE, Hahm S, Marks JD, Reiter RE, Wu AM An affinity matured minibody for PET imaging of prostate stem cell antigen (PSCA)-expressing tumors. Eur J Nucl Med Mol Imaging. 2010; 37(8): 1529-38.
Barat B, Kenanova VE, Olafsen T, Wu AM Evaluation of Two Internalizing Carcinoembryonic Antigen Reporter Genes for Molecular Imaging. Mol Imaging Biol. 2010; Epub ahead of print: .
Olafsen T, Sirk SJ, Betting DJ, Kenanova VE, Bauer KB, Ladno W, Raubitschek AA, Timmerman JM, Wu AM ImmunoPET imaging of B-cell lymphoma using 124I-anti-CD20 scFv dimers (diabodies). Protein Eng Des Sel. 2010; 23(4): 243-9..
McCabe KE, Wu AM Positive progress in immunoPET--not just a coincidence. Cancer Biother Radiopharm. 2010; 25(3): 253-61.
Aweda TA, Beck HE, Wu AM, Wei LH, Weber WA, Meares CF Rates and equilibria for probe capture by an antibody with infinite affinity. Bioconjug Chem. 2010; 21(4): 784-91.
Kenanova VE, Olafsen T, Salazar FB, Williams LE, Knowles S, Wu AM Tuning the serum persistence of human serum albumin domain III:diabody fusion proteins. Protein Eng Des Sel. 2010; 23(10): 789-98.
Olafsen T, Young SG, Davies BS, Beigneux AP, Kenanova VE, Voss C, Young G, Wong KP, Barnes RH 2nd, Tu Y, Weinstein MM, Nobumori C, Huang SC, Goldberg IJ, Bensadoun A, Wu AM, Fong LG Unexpected expression pattern for glycosylphosphatidylinositol-anchored HDL-binding protein 1 (GPIHBP1) in mouse tissues revealed by positron emission tomography scanning. J Biol Chem. 2010; 285(50): 39239-48.
Sherman David J, Kenanova Vania E, Lepin Eric J, McCabe Katelyn E, Kamei Ken-Ichiro, Ohashi Minori, Wang Shutao, Tseng Hsian-Rong, Wu Anna M, Behrenbruch Christian P A differential cell capture assay for evaluating antibody interactions with cell surface targets. Analytical biochemistry. 2010; 401(2): 173-81.
McCabe Katelyn E, Wu Anna M Positive progress in immunoPET--not just a coincidence. Cancer biotherapy & radiopharmaceuticals. 2010; 25(3): 253-61.
Olafsen Tove, Wu Anna M Antibody vectors for imaging. Seminars in nuclear medicine. 2010; 40(3): 167-81.
Olafsen Tove, Sirk Shannon J, Betting David J, Kenanova Vania E, Bauer Karl B, Ladno Waldemar, Raubitschek Andrew A, Timmerman John M, Wu Anna M ImmunoPET imaging of B-cell lymphoma using 124I-anti-CD20 scFv dimers (diabodies). Protein engineering, design & selection : PEDS. 2010; 23(4): 243-9.
Gagnon Pete, Cheung Chia-Wei, Lepin Eric J, Wu Anna M, Sherman Mark A, Raubitschek Andrew A, Yazaki Paul J Minibodies and Multimodal Chromatography Methods: A Convergence of Challenge and Opportunity. BioProcess international. 2010; 8(2): 26-35.
Olafsen Tove, Betting David, Kenanova Vania E, Salazar Felix B, Clarke Pat, Said Jonathan, Raubitschek Andrew A, Timmerman John M, Wu Anna M Recombinant anti-CD20 antibody fragments for small-animal PET imaging of B-cell lymphomas. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 2009; 50(9): 1500-8.
Barat Bhaswati, Sirk Shannon J, McCabe Katelyn E, Li Jianqing, Lepin Eric J, Remenyi Roland, Koh Ai Leen, Olafsen Tove, Gambhir Sanjiv S, Weiss Shimon, Wu Anna M Cys-diabody quantum dot conjugates (immunoQdots) for cancer marker detection. Bioconjugate chemistry. 2009; 20(8): 1474-81.
Vaidyanathan Ganesan, Jestin Emmanuelle, Olafsen Tove, Wu Anna M, Zalutsky Michael R Evaluation of an anti-p185(HER2) (scFv-C(H)2-C(H)3)2 fragment following radioiodination using two different residualizing labels: SGMIB and IB-Mal-D-GEEEK. Nuclear medicine and biology. 2009; 36(6): 671-80.
Wu, A. M. Antibodies and Antimatter: The Resurgence of Immuno-PET. <a href="http://jnm.snmjournals.org/cgi/content/full/50/1/2">Journal of Nuclear Medicine</a> 2009; 50(1): 2-5.
Shimazaki, K. Chan, A. M. Moniz, R. J. Wadehra, M. Nagy, A. Coulam, C. P. Mareninov, S. Lepin, E. M. Wu, A. M. Kelly, K. A. Braun, J. Gordon, L. K. Blockade of epithelial membrane protein 2 (EMP2) abrogates infection of Chlamydia muridarum murine genital infection model. Fems Immunology and Medical Microbiology. 2009; 55(2): 240-249.
Leyton, J.V., Olafsen, T., Sherman, M.A., Bauer, K.B., Aghajanian, P., Reiter, R.E., and Wu, A.M. Engineered humanized diabodies for microPET imaging of prostate stem cell antigen (PSCA)-expressing tumors. Protein Engineering Design & Selection. 2009; 22: 209-216.
Schipper, M. L. Iyer, G. Koh, A. L. Cheng, Z. Ebenstein, Y. Aharoni, A. Keren, S. Bentolila, L. A. Li, J. Q. Rao, J. H. Chen, X. Y. Banin, U. Wu, A. M. Sinclair, R. Weiss, S. Gambhir, S. S. Particle Size, Surface Coating, and PEGylation Influence the Biodistribution of Quantum Dots in Living Mice. Small. 2009; 5(1): 126-134.
Olafsen T, Betting D, Kenanova VE, Salazar FB, Clarke P, Said J, Raubitschek AA, Timmerman JM, Wu AM Recombinant anti-CD20 antibody fragments for small-animal PET imaging of B-cell lymphomas. J Nucl Med. 2009; 50(9): 1500-8.
Afshar Sepideh, Olafsen Tove, Wu Anna M, Morrison Sherie L Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT. Journal of experimental & clinical cancer research : CR. 2009; 28(12): 147.
Frank Richard T, Edmiston Marissa, Kendall Stephen E, Najbauer Joseph, Cheung Chia-Wei, Kassa Thewodros, Metz Marianne Z, Kim Seung U, Glackin Carlotta A, Wu Anna M, Yazaki Paul J, Aboody Karen S Neural stem cells as a novel platform for tumor-specific delivery of therapeutic antibodies. PloS one. 2009; 4(12): e8314.
Leyton, J. V. Olafsen, T. Lepin, E. J. Hahm, S. Bauer, K. B. Reiter, R. E. Wu, A. M. Humanized Radioiodinated Minibody For Imaging of Prostate Stem Cell Antigen-Expressing Tumors. Clinical Cancer Research. 2008; 14(22): 7488-7496.
Wu, A. M. Olafsen, T. Antibodies for molecular imaging of cancer. Cancer Journal. 2008; 14(3): 191-197.
Wu, A. M. Sirk, S. Barat, B. Olafsen, T. Cys-diabodies for nanoparticle conjugation and targeting. Cancer Biotherapy and Radiopharmaceuticals 2008; 23(4): 514-514.
Shimazaki, K., Lepin, E.J., Wei, B., Nagy, A., Coulum, P., Mareninov, S., Fu, M., Wu, A.M., Marks, J.D., Braun, J., Gordon, L.K., and Wadehra, M. Diabodies targeting epithelial membrane protein 2 reduce tumorigenicity of human endometrial cancer cell lines. Clin. Cancer Res. 2008; 14: 7367-7377.
Wei, L. H. Olafsen, T. Radu, C. Hildebrandt, I. J. Mccoy, M. R. Phelps, M. E. Meares, C. Wu, A. M. Czernin, J. Weber, W. A. Engineered Antibody Fragments with Infinite Affinity as Reporter Genes for PET Imaging. Journal of Nuclear Medicine. 2008; 49(11): 1828-1835.
Kenanova, V.E., Barat, B., Olafsen, T., Chatziioannou, A., Herschman, H.R., Braun, J., and Wu, A.M. Recombinant carcinoembryonic antigen as a reporter gene for molecular imaging. Eur. J. Nucl. Med. Molec. Imaging. 2008; 36: 104-114.
Sirk, S., Olafsen, T., Barat, B., Bauer, K. B., McCabe, K.E., and Wu, A.M. Site-specific thiol-mediated conjugation of fluorescent probes to cysteine-modified diabodies targeting CD20 or HER2. Bioconjugate Chemistry. 2008; 19: 2527-2534.
Loening, AM Wu, AM Gambhir, SS Red-shifted Renilla reniformis luciferase variants for imaging in living subjects. Nature Methods. 2007; 4(8): 641-3.
Venisnik, K. M. Olafsen, T. Gambhir, S. S. Wu, A. M. Fusion of Gaussia luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imaging. Molecular Imaging and Biology. 2007; 9(5): 267-277.
Barat, B. Wu, A. M. Metabolic biotinylation of recombinant antibody by biotin ligase retained in the endoplasmic reticulum. Biomolecular Engineering. 2007; 24(3): 283-291.
Schipper, M.L., Cheng, Z., Lee, S.-W., Bentolila, L.A., Iyer, G., Rao, J., Chen, X., Wu, A.M., Weiss, S., and Gambhir, S.S. MicroPET-based biodistribution of quantum dots in living mice. J. Nucl. Med. 2007; 48: 1511-1518.
Olafsen, T. Gu, Z. Sherman, M. A. Leyton, J. V. Witkosky, M. E. Shively, J. E. Raubitschek, A. A. Morrison, S. L. Wu, A. M. Reiter, R. E. Targeting, imaging, and therapy using a humanized antiprostate stem cell antigen (PSCA) antibody. Journal of Immunotherapy. 2007; 30(4): 396-405.
Cai, W Olafsen, T Zhang, X Cao, Q Gambhir, SS Williams, LE Wu, AM Chen, X PET imaging of colorectal cancer in xenograft-bearing mice by use of an 18F-labeled T84.66 anti-carcinoembryonic antigen diabody. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 2007; 48(2): 304-10.
Kenanova, V. Olafsen, T. Williams, L. E. Ruel, N. H. Longmate, J. Yazaki, P. J. Shively, J. E. Colcher, D. Raubitschek, A. A. Wu, A. M. Radioiodinated versus radiometal-labeled anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments: Optimal pharmacokinetics for therapy. Cancer Research. 2007; 67(2): 718-726.
Sui, G. D. Wang, J. Y. Lee, C. C. Lu, W. X. Lee, S. P. Leyton, J. V. Wu, A. M. Tseng, H. R. Solution-phase surface modification in intact poly(dimethylsiloxane) microfluidic channels. Analytical Chemistry. 2006; 78(15): 5543-5551.
Wong, J.Y.C., Chu, D.Z., Williams, L.E., Liu, A., Zhan, J., Yamauchi, D.M., Wilczynski, S., Wu, A.M., Yazaki, P.J., Shively, J.E., Leong, L., Raubitschek, A.A. A Phase I trial of 90Y-DOTA-anti-CEA chimeric T84.66 (cT84.66) radioimmunotherapy in patients with metastatic CEA-producing malignancies. Cancer Biother. Radiopharm. 2006; 21: 88-100.
Ferl, G. Z. Kenanova, V. Wu, A. M. DiStefano, J. J. A two-tiered physiologically based model for dually labeled single-chain Fv-Fc antibody fragments. Molecular Cancer Therapeutics. 2006; 5(6): 1550-1558.
Leyton, J. V. Olafsen, T. Sherman, M. A. Reiter, R. E. Wu, A. M. Anti-prostate stem cell antigen (PSCA) antibody fragments for pet imaging of prostate cancer. Cancer Biotherapy and Radiopharmaceuticals. 2006; 21(4): 391-391.
Venisnik, K. M. Olafsen, T. Loening, A. M. Iyer, M. Gambhir, S. S. Wu, A. M. Bifunctional antibody-Renilla luciferase fusion protein for in vivo optical detection of tumors. Protein Engineering Design & Selection. 2006; 19(10): 453-460.
Loening, A. M. Fenn, T. D. Wu, A. M. Gambhir, S. S. Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output. Protein Engineering Design & Selection. 2006; 19(9): 391-400.
Li, L. Yazaki, P. J. Anderson, A. L. Crow, D. Colcher, D. Wu, A. M. Williams, L. E. Wong, J. Y. C. Raubitschek, A. Shively, J. E. Improved biodistribution and radioimmunoimaging with poly(ethylene glycol)-DOTA-conjugated anti-CEA diabody. Bioconjugate Chemistry. 2006; 17(1): 68-76.
Olafsen, T. Kenanova, V. E. Leyton, J. V. Betting, D. Raubitschek, A. A. Timmerman, J. M. Reiter, R. E. Wu, A. M. Micropet evaluation of an I-124-labeled antibody fragment (scFv-Fc) in non-internalizing (CEA and CD20) versus internalizing (HER2 and PSCA) tumor antigen systems. Cancer Biotherapy and Radiopharmaceuticals. 2006; 21(4): 391-391.
Kenanova, V.E. and Wu, A.M. Tailoring antibodies for radionuclide delivery. Exp. Opin. Drug Delivery. 2006; 3: 53-70.
Olafsen, T Kenanova, VE Wu, AM Tunable pharmacokinetics: modifying the in vivo half-life of antibodies by directed mutagenesis of the Fc fragment. Nature protocols. 2006; 1(4): 2048-60.
Sui, G. D. Wang, J. Zeta, T. F. Y. Leyton, J. V. Wu, A. M. Tseng, H. R. Surface modification of PDMS-surfaces for protein-passivation, cell immobilization, DNA array and immunoassay. Abstracts of Papers of the American Chemical Society 2006; 231: .
Olafsen, T. Kenanova, V. E. Sundaresan, G. Anderson, A. L. Crow, D. Yazaki, P. J. Li, L. Press, M. F. Williams, L. E. Wong, J. Y. C. Raubitschek, A. A. Shively, J. E. Wu, A. M. Optimizing radiolabeled engineered anti-p185(HER2) antibody fragments for in vivo imaging. Cancer Research. 2005; 65(13): 5907-5916.
Ferl, G. Z. Wu, A. M. Distefano, J. J. A predictive model of therapeutic monoclonal antibody dynamics and regulation by the neonatal fc receptor (FcRn). Annals of Biomedical Engineering. 2005; 33(11): 1640-1652.
Huang, S. C. Truong, D. Wu, H. M. Chatziioannou, A. F. Shao, W. Wu, A. M. Phelps, M. E. An Internet-based "kinetic imaging system" (KIS) for MicroPET. Molecular Imaging and Biology. 2005; 7(5): 330-341.
Wu, A. M. Senter, P. D. Arming antibodies: prospects and challenges for immunoconjugates. Nature Biotechnology. 2005; 23(9): 1137-1146.
Michalet, X. Pinaud, F. F. Bentolila, L. A. Tsay, J. M. Doose, S. Li, J. J. Sundaresan, G. Wu, A. M. Gambhir, S. S. Weiss, S. Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 2005; 307(5709): 538-544.
Kenanova, V. Olafsen, T. Crow, D. M. Sundaresan, G. Subbarayan, M. Carter, N. H. Ikle, D. N. Yazaki, P. J. Chatziioannou, A. F. Gambhir, S. S. Williams, L. E. Shively, J. E. Colcher, D. Raubitschek, A. A. Wu, A. M. Tailoring the pharmacokinetics and positron emission tomography imaging properties of anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments. Cancer Research. 2005; 65(2): 622-631.
Wong, J. Y. C. Chu, D. Z. Williams, L. E. Yamauchi, D. M. Ikle, D. N. Kwok, C. S. Liu, A. Wilczynski, S. Colcher, D. Yazaki, P. J. Shively, J. E. Wu, A. M. Raubitschek, A. A. Pilot trial evaluating an I-123-labeled 80-kilodalton engineered anticarcinoembryonic antigen antibody fragment (cT84.66 minibody) in patients with colorectal cancer. Clinical Cancer Research. 2004; 10(15): 5014-5021.
Yazaki, P. J. Sherman, M. A. Shively, J. E. Ikle, D. Williams, L. E. Wong, J. Y. C. Colcher, D. Wu, A. M. Raubitschek, A. A. Humanization of the anti-CEA T84.66 antibody based on crystal structure data. Protein Engineering Design & Selection. 2004; 17(5): 481-489.
Olafsen T, Tan GJ, Cheung CW, Yazaki PJ, Park JM, Shively JE, Williams LE, Raubitschek AA, Press MF, Wu AM Characterization of engineered anti-p185HER-2 (scFv-CH3)2 antibody fragments (minibodies) for tumor targeting. Protein engineering, design & selection : PEDS. . 2004; 17(4): 315-23.
Olafsen T, Cheung CW, Yazaki PJ, Li L, Sundaresan G, Gambhir SS, Sherman MA, Williams LE, Shively JE, Raubitschek AA, Wu AM Covalent disulfide-linked anti-CEA diabody allows site-specific conjugation and radiolabeling for tumor targeting applications. Protein engineering, design & selection : PEDS. . 2004; 17(1): 21-7.
Sundaresan G, Yazaki PJ, Shively JE, Finn RD, Larson SM, Raubitschek AA, Williams LE, Chatziioannou AF, Gambhir SS, Wu AM 124I-labeled engineered anti-CEA minibodies and diabodies allow high-contrast, antigen-specific small-animal PET imaging of xenografts in athymic mice. Journal of nuclear medicine: Official Publication, Society of Nuclear Medicine. 2003; 44(12): 1962-9.
Jensen MC, Cooper LJ, Wu AM, Forman SJ, Raubitschek A Engineered CD20-specific primary human cytotoxic T lymphocytes for targeting B-cell malignancy. Cytotherapy. . 2003; 5(2): 131-8.
Olafsen, T. Tan, G. J. Park, J. M. Crow, D. Li, L. Sundaresan, G. Gambhir, S. S. Shively, J. E. Raubitschek, A. A. Press, M. F. Wu, A. M. Tumor targeting, biodistribution and micro-PET imaging of engineered anti-HER2/neu antibody fragments in xenograft-bearing mice. Journal of Nuclear Medicine 2003; 44(5): 363p-363p.
Ray, P. Wu, A. M. Gambhir, S. S. Optical bioluminescence and positron emission tomography imaging of a novel fusion reporter gene in tumor xenografts of living mice. Cancer Research. 2003; 63(6): 1160-1165.
Carmichael JA, Power BE, Garrett TP, Yazaki PJ, Shively JE, Raubischek AA, Wu AM, Hudson PJ The crystal structure of an anti-CEA scFv diabody assembled from T84.66 scFvs in V(L)-to-V(H) orientation: implications for diabody flexibility. J Mol Biol. 2003; 326(2): 341-51.
Sundaresan, G. Yazaki, P. J. Shively, J. E. Toyokuni, T. Nguyen, K. N. Finn, R. Larson, S. M. Raubitschek, A. A. Gambhir, S. S. Wu, A. M. I-124 radiolabeled genetically engineered anti-CEA antibody fragments for tumor imaging with microPET. Journal of Nuclear Medicine 2001; 42(5): 153p-153p.
Abdel-Jalil, R. J. Walsh, J. C. Stone, J. L. Shively, J. E. Satyamurthy, N. Barrio, J. R. Phelps, M. E. Wu, A. M. Gambhir, S. S. Toyokuni, T. Synthesis of 4-[(4-(18)Ffluorobenzylidene) aminooxy]butyl vinyl sulfone as an amine- and thiol-reactive F-18-labeling agent. Journal of Nuclear Medicine 2001; 42(5): 256p-256p.
Wu, A. M. Tools for pretargeted radioimmunotherapy. Cancer Biotherapy and Radiopharmaceuticals. 2001; 16(2): 103-108.
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